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Registros recuperados: 6
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AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development Genet. Mol. Biol.
Gerhardt,Liliane B. de A.; Magioli,Cláudia; Perez,Ana B.U.C.M.; Margis,Rogério; Sachetto-Martins,Gilberto; Margis-Pinheiro,Márcia.
The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Chia4 Arabidopsis thaliana endochitinase; Embryogenesis; Gene expression; Promoter.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020
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Classification, expression pattern and comparative analysis of sugarcane expressed sequences tags (ESTs) encoding glycine-rich proteins (GRPs) Genet. Mol. Biol.
Fusaro,Adriana; Mangeon,Amanda; Junqueira,Ricardo Magrani; Rocha,Carla Andréa Benício; Coutinho,Tatiana Cardoso; Margis,Rogério; Sachetto-Martins,Gilberto.
Since the isolation of the first glycine-rich proteins (GRPs) in plants a wealth of new GRPs have been identified. The highly specific but diverse expression pattern of grp genes, taken together with the distinct sub-cellular localization of some GRP groups, clearly indicate that these proteins are involved in several independent physiological processes. Notwithstanding the absence of a clear definition of the role of GRPs in plant cells, studies conducted with these proteins have provided new and interesting insights into the molecular biology and cell biology of plants. Complexly regulated promoters and distinct mechanisms for the regulation of gene expression have been demonstrated and new protein targeting pathways, as well as the exportation of GRPs...
Tipo: Info:eu-repo/semantics/article
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100035
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In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.) Rev. Bras. Bot.
Tarré,Erika; Magioli,Claudia; Margis-Pinheiro,Márcia; Sachetto-Martins,Gilberto; Mansur,Elisabeth; Santiago-Fernandes,Lygia D. R..
Somatic embryogenesis was induced from cotyledon explants of eggplant cultured on MS medium supplemented with 54 µM NAA. Anatomical analysis of somatic embryo initiation and development was performed during the first four weeks. Proembryo formation was observed after the second day of culture, directly from perivascular cells or via pro-embryogenic masses derived from indeterminate meristematic masses (IMMs) originated in the vascular tissue. Those IMMs also gave rise to root primordia after 10 days of culture. The origin of embryos is discussed as well as the similarities between somatic embryogenesis and adventitious root formation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Development; Solanum melongena; Somatic embryogenesis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042004000100010
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Morfogênese in vitro de variedades brasileiras de cana-de-açúcar PAB
Cidade,Daniela Anhel de Paula; Garcia,Renata de Oliveira; Duarte,Aline Castellar; Sachetto-Martins,Gilberto; Mansur,Elisabeth.
O objetivo deste trabalho foi estabelecer sistemas de multiplicação de plantas de cana-de-açúcar in vitro e avaliar sua utilização, como material inicial, para a indução de regeneração a partir de ápices caulinares. Três métodos de cultivo foram avaliados: cultura em meio semi-sólido, cultura líquida estacionária e cultura líquida sob agitação. A taxa de multiplicação mais elevada foi alcançada por meio da cultura líquida sob agitação. Ápices caulinares, excisados dessas plantas, apresentaram taxas de regeneração in vitro compatíveis com sua utilização em protocolos de transformação. Calos resistentes a PPT e GUS-positivos foram obtidos de explantes da variedade Chunnee com inoculação de Agrobacterium tumefaciens C58C1 (pMP90) (pDUBarA9). O protocolo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum; Micropropagação; Transformação genética; Agrobacterium tumefaciens.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000300003
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Sugarcane expressed sequences tags (ESTs) encoding enzymes involved in lignin biosynthesis pathways Genet. Mol. Biol.
Ramos,Rose Lucia Braz; Tovar,Francisco Javier; Junqueira,Ricardo Magrani; Lino,Fabiane Borges; Sachetto-Martins,Gilberto.
Lignins are phenolic polymers found in the secondary wall of plant conductive systems where they play an important role by reducing the permeability of the cell wall to water. Lignins are also responsible for the rigidity of the cell wall and are involved in mechanisms of resistance to pathogens. The metabolic routes and enzymes involved in synthesis of lignins have been largely characterized and representative genes that encode enzymes involved in these processes have been cloned from several plant species. The synthesis of lignins is liked to the general metabolism of the phenylpropanoids in plants, having enzymes (e.g. phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H) and caffeic acid O-methyltransferase (COMT)) common to other processes...
Tipo: Info:eu-repo/semantics/article
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100031
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Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database (ForEST) Genet. Mol. Biol.
Bocca,Silvia Nora; Magioli,Claudia; Mangeon,Amanda; Junqueira,Ricardo Magrani; Cardeal,Vanessa; Margis,Rogério; Sachetto-Martins,Gilberto.
The occurrence of quasi-repetitive glycine-rich peptides has been reported in different organisms. Glycine-rich regions are proposed to be involved in protein-protein interactions in some mammalian protein families. In plants, a set of glycine-rich proteins (GRPs) was characterized several years ago, and since then a wealth of new GRPs have been identified. GRPs may have very diverse sub-cellular localization and functions. The only common feature among all different GRPs is the presence of glycine-rich repeat domains. The expression of genes encoding GRPs is developmentally regulated, and also induced, in several plant genera, by physical, chemical and biological factors. In addition to the highly modulated expression, several GRPs also show...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine-rich; GRP; Eucalyptus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400016
Registros recuperados: 6
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